Dr Baldev Chadha (Annu Kapoor), a well qualified fertility expert, runs a fertility clinic and a sperm bank in Daryaganj, Delhi, that guarantees a high quality and specialized sperm for the purpose. Unfortunately he has more failed cases to his credit than successes. A healthy, high performing donor is the need of the hour. Vicky Arora (Ayushmann Khurrana), who is the only son and of no financial support to Dolly, a widow, who runs a small beauty parlor from home in Lajpat Nagar.

As luck would have it, a small brawl in the colony brings Dr Chadha and Vicky, face to face, where Chadha , concludes that Vicky could be THAT donor he has been looking for. From here on, Chadha's days and nights are spent in convincing Vicky to become a donor till he finally gives in. Vicky finds his love interest in Ashima Roy (Yami Gautam), a pretty Bengali girl, who works in a Bank. But soon their hunky dory world comes crashing down because of Vicky's past as a donor. With a brief emotional roller coaster ride, Vicky Donor is a light-hearted take on the subject of sperm donation